Table of Contents
What is MDBK cell?
Madin-Darby bovine kidney (MDBK) cells are a suitable cell line for the propagation and study of the bovine poxvirus lumpy skin disease virus.
What is Vero cell culture?
Vero cells are derived from the kidney of an African green monkey, and are one of the more commonly used mammalian continuous cell lines in microbiology, and molecular and cell biology research. This unit includes protocols for the growth and maintenance of Vero cell lines in a research laboratory setting.
Why are MDCK cells used?
Because of their high susceptibility to infection with various influenza virus strains, Madin-Darby canine kidney (MDCK) cells have been widely used as a substrate for influenza virus isolation and vaccine production.
What are MDCK cells used for?
MDCK cells are used for a wide variety of cell biology studies including cell polarity, cell-cell adhesions (termed adherens junctions), collective cell motility, as well as responses to growth factors.
What are calu 3 cells?
Calu-3 is a non-small-cell lung cancer cell line that grows in adherent culture and displays epithelial morphology. These cells have constitutively active ErbB2/Her2 due to amplification of the ERBB2 gene.
What are C6 36 cells?
Background. The 50-year-old Aedes albopictus C6/36 cell line is a resource for the detection, amplification, and analysis of mosquito-borne viruses including Zika, dengue, and chikungunya. The cell line is derived from an unknown number of larvae from an unspecified strain of Aedes albopictus mosquitoes.
How do you freeze MDCK cells?
Freeze the cells at a cooling rate between 1-3oC/min using a programmable rate controlled freezer or suitable alternative1. When the temperature reaches at least -130oC, transfer the ampoule to a gas phase liquid nitrogen storage vessel.
What type of cells are MDCK?
The cells exhibit resistance to coxsackievirus B3 and B4 as well as poliovirus 2, and are negative for reverse transcriptase. The MDCK line is commonly used as a general model for epithelial cells, which comprise the type of tissue known as epithelium.
What does calu 3 stand for?
Cultured Human Airway Epithelial Cells
Cultured Human Airway Epithelial Cells (Calu-3): A Model of Human Respiratory Function, Structure, and Inflammatory Responses.
What are BEAS 2B cells?
The BEAS-2B cell line was originally established as a human non-tumorigenic lung epithelial cell line derived from a human lung tissue and has been extensively used as an in vitro non-tumorigenic lung epithelial model in a large variety of studies in association with lung carcinogenesis for over 30 years.
What kind of tissue is c6 36 cells derived from?
larvae
ECACC General Cell Collection: Clone C6/36
| Supplied by: | European Collection of Authenticated Cell Cultures (ECACC) |
|---|---|
| Species: | Insect |
| Tissue of Origin: | larvae |
| Growth Mode: | Adherent |
| Karyotype: | Diploid |
Why is DMSO used to freeze cells?
When added to media, DMSO prevents intracellular and extracellular crystals from forming in cells during the freezing process. Without a cryoprotectant, these crystals cause cell death, thus rendering the cells useless for transplant. DMSO is almost always used in the banking of cord blood cells.
Can you freeze cells without DMSO?
Remember, DMSO is a universal solvent. So anything less than almost pure, and you are dealing with unknowns that could harm your cells. Cells survive better if you re-suspend them in cold freezing medium.
What is A549 cell line?
A549 cells are lung carcinoma epithelial cells that constitute a cell line. A549 is one of the commonly used cell lines for a wide range of research applications.
What are calu 1 cells?
Description. Calu-1 is a non-small-cell lung cancer (NSCLC) cell line that grows in adherent culture and displays epithelial morphology. These cells express wildtype LKB1, wildtype EGFR, and mutant K-Ras (G12C).
What is C6 36 cell line?
The 50-year-old Aedes albopictus C6/36 cell line is a resource for the detection, amplification, and analysis of mosquito-borne viruses including Zika, dengue, and chikungunya. The cell line is derived from an unknown number of larvae from an unspecified strain of Aedes albopictus mosquitoes.
How do you isolate dengue virus?
Out of the 97 dengue patients, dengue virus was isolated from 44 (45.4%) cases by the mosquito inoculation technique and from 24 (24.7%) cases by cell culture (Table 1). The rate of dengue virus isolation was significantly higher by mosquito inoculation than by cell culture inoculation technique (p<0.01).
How long can cells stay in DMSO?
Cells are allowed to absorb DMSO into cell membranes for 15 minutes. The DMSO acts to prevent ice crystal formation during the slow freezing process, maintaining cell viability. Some cell types such as PBMC may be stored short term (less than one week) at -800 C or long term in a liquid nitrogen freezer.
How do you dilute DMSO for cell culture?
1-0.2% DMSO as a final concentration. Then if you have 1 ml volume, you can add 1-2ul of your compound directly into the 1 ml. I don’t remember what the maximum concentration your cells can tolerate, but I would use that concentration and work backwards to determine how many ul you need to add.
How long can cells survive in DMSO?
This may be in some cases associated with a reversible cell cycle arrest (CHO cells, 1-2% DMSO, 96 hrs), and in other cases with cell differentiation to other lineages (HL-60 cells, 1.3% DMSO, 96 hrs) or cell death at even higher concentrations of DMSO (Caco-2 cells, >10% DMSO, the release of LDH).