Table of Contents
What is a phosphorothioate oligonucleotide?
In this context phosphorothioate oligonucleotides are compounds addressed to hybridize to a targeted mRNA inducing a variety of effects including inhibition of the expression of proteins involved in different pathological processes and preventing translation.
What are antisense oligodeoxynucleotides?
Antisense oligonucleotides (ASOs) are short, synthetic, chemically modified chains of nucleotides that have the potential to target any gene product of interest. Typically, an ASO is a single-stranded sequence complementary to the sequence of the target gene’s transcribed messenger RNA (mRNA) within a cell.
What is phosphorothioate bond?
Phosphorothioate Bond The phosphorothioate (PS) bond substitutes a sulfur atom for a non-bridging oxygen in the phosphate backbone of an oligo. This modification renders the internucleotide linkage resistant to nuclease degradation.
What is the difference between the sense and antisense strand?
Sense strand contains the exact nucleotide sequence to the mRNA which encodes for a functional protein. Antisense strand serves as the template for the transcription, and contains complementary nucleotide sequence to the transcribed mRNA. Therefore, antisense strand is responsible for translating proteins.
What is the main purpose of phosphorothioate bonds modifications?
The phosphorothioate (PS) bond substitutes a sulfur atom for a non-bridging oxygen in the phosphate backbone of an oligo. This modification renders the internucleotide linkage resistant to nuclease degradation.
Is mRNA sense or antisense?
Strictly speaking, only the mRNA makes “sense” with the genetic code, as the translated protein peptide sequence can be directly inferred from this strand. The “antisense” strand of DNA is complementary to the “sense” strand and is the actual template for mRNA synthesis.
What is the difference between sense strand and antisense strand?
The sense strand has the information that would be readable on the RNA, and that’s called the coding side. The antisense is the non-coding strand, but ironically, when you’re making RNA, the proteins that are involved in making RNA read the antisense strand in order to create a sense strand for the mRNA.
What are sense and antisense strands?
Main Difference – Sense vs Antisense Strand Sense strand contains the exact nucleotide sequence to the mRNA which encodes for a functional protein. Antisense strand serves as the template for the transcription, and contains complementary nucleotide sequence to the transcribed mRNA.
What are antisense Morpholinos?
A Brief Introduction to Morpholino Antisense. Morpholino oligos are advanced tools for blocking sites on RNA to obstruct cellular processes. A Morpholino oligo specifically binds to its selected target site to block access of cell components to that target site.
Can phosphorothioate create oligonucleotides for antisense knockdown?
These features make phosphorothioate effective in creating oligonucleotides for antisense knockdowns, one of the early and still-pursued nucleic acid therapeutic technologies 2.
What is phosphorothioate used for in biotechnology?
Phosphorothioate is effective at reducing nucleolytic degradation of oligonucleotides needed for both in vivo and in vitro technologies. Several synthesis scales and purifications are available to meet a variety of research and applied needs.
What are phosphorothioate oligonucleotides?
In this context phosphorothioate oligonucleotides are compounds addressed to hybridize to a targeted mRNA inducing a variety of effects including inhibition of the expression of proteins involved in different pathological processes and preventing translation.
What is the best way to purify phosphorothioate oligonucleotides?
Phosphorothioate oligonucleotides are amenable to purification via HPLC, which is beneficial since they are often used in vivo and therefore must be of the highest homogeneity possible to avoid cytotoxic effects, off-target binding, etc.